Anti-inflammatory potentials of ethanolic leaf extract of Gongronema latifolium in streptozotocin induced diabetic rats
1 Department of Human Physiology, Cross River University of Technology, Okuku Campus, Cross River State, Nigeria.
2 Department of Human Physiology, University of Calabar, Cross River State, Nigeria.
3 Department of Physiology PAMO University of Medical Sciences, Port Harcourt, Rivers State, Nigeria.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2022, 11(01), 077–084.
Article DOI: 10.30574/wjbphs.2022.11.1.0109
Publication history:
Received on 22 June 2022; revised on 28 July 2022; accepted on 30 July 2022
Abstract:
Introduction: Inflammation due to diabetes mellitus has been understudied. Gongronema latifolium (GL) is reported to have anti-inflammatory potentials. This work was designed to evaluate the effect of ethanolic leaf extract of Gongronema latifolium on some inflammatory markers in Streptozotocin (STZ)-induced diabetic rats.
Materials and methods: 40 Wistar rats of both sexes (150 g-200 g) were divided into 5 groups of eight (8) rats each. Group 1; control received normal saline placebo orally. Group 2; GL only received 200 mg/kg of GL orally. Group 3 DM only received 65 mg/kg bw of STZ intraperitoneally for two days with an interval of one day in between. Group 4; DM + GL received 65 mg/kg bw of STZ + 200mg/kg bw of GL. Group 5; DM + Insulin received 65mg/kgbw of STZ followed by 10 IU/kgbw of insulin subcutaneously. For further inflammatory study, paw oedema was induced by sub-plantar injection of 2% formalin in two groups before and after administration of 200 mg/kg bw of GL daily for 7 days. Blood samples were collected via cardiac puncture for biochemical analysis.
Results: Results from this study showed a significant (p<0.05) increase in blood glucose DM group, compared to the control. Intervention with GL and insulin significantly reduced the glucose level towards normal. Interleukin-6 concentration and C-reactive proteins were significantly (p<0.01) higher in DM group compared with the GL and control groups. Paw oedema size was significantly (p<0.05) increased in the formalin treated group compared with the control. Pretreatment and post treatment with GL significantly (p<0.05) reduced the paw oedema size.
Conclusion: In conclusion, GL (like insulin) has hypoglycemic and anti-inflammatory potentials.
Keywords:
Gongronema latifolium; Diabetes; C-reactive peptide; Interleukin-6; Paw oedema
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