Antihypertensive activity of Moringa oleifera seeds hydroalcoholic extract on high sodium diet-induced hypertension in Wistar Rat

Donné Yodan RALAHIRAVO 1, *, Olivier RAKOTOARISON 1, Fanantenanirainy RANDIMBIVOLOLONA 1, Nat QUANSAH 2 and Patricia RANDRIANAVONY 1

1 Pharmacology Department, Faculty of Sciences, University of Antananarivo, Madagascar.
2 School for International Training (SIT) Study Abroad Faculty of Sciences, University of Antananarivo, Madagascar.
 
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 13(02), 350–356.
Article DOI: 10.30574/wjbphs.2023.13.2.0110
Publication history: 
Received on 12 January 2023; revised on 24 February 2023; accepted on 27 February 2023
 
Abstract: 
Our objective was to study the effect of hydroalcoholic extract of M. oleifera seeds on high blood pressure in Wistar rat. After 21 days of a high sodium diet, the value of systolic blood pressure and diastolic blood pressure increased from 87. 2 ± 1.96 / 58 ± 0.89 mm to 200.8 ± 0.49 /151.2 ± 1.2 mm Hg (p < 0.05). Administered orally, the extract reduces the high blood pressure of hypertensive rats. It returns to its normal value after 19 days for the control batch, versus 9, 6, and 3 days for the animals treated with the extract at doses 100, 200, and 400 mg/kg (P < 0.05). The extract also has a diuretic activity; the 24 h urinary volume increases from 4.8 ± 0.14 ml in control group, versus 6.85 ± 0.1, 8.3 ± 0.13 and 10.7 ± 0.5 ml in groups treated with the extract at doses 100, 200 and 400 mg/kg (p < 0.05), while natriuresis increases from 2.06 ± 0.05 mEq/L in control group, versus 2.92 ± 0.05; 6.82 ± 0.11 and 10.73 ± 0.17 mEq/L in animals treated with the extract at doses 100, 200 and 400 mg/kg (p < 0.05). In vitro, it relaxes the isolated aorta contracted with norepinephrine at the concentration of 10-5 M in the bath, with EC50 equal to 2.06 mg/ml (p < 0.05). These results show that the hydroalcoholic extract of M. oleifera seeds has antihypertensive activity through its diuretic and vasodilator effects.
 
Keywords: 
Moringa oleifera; Hypertension; Diuretic; Vasodilation; Rat
 
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0