Bacteriological Profile and Antibiotic Sensitivity Patterns of Bacterial Isolates from Pus/Wound Swab

The existence of high drug-resistance bacterial wound infection is a serious problem, especially in surgical practice. The inappropriate use of antibiotics has resulted in the development of antibiotic resistance. The bacteriological profile may remain the same, but the antibiotic susceptibility pattern varies. 
Objective: The main objective of the study is to identify the bacteriological profile of pus/wound swab samples and their antibiotic susceptibility pattern. This retrospective study was carried out from August 2019 to January 2020. A total of 300 clinical specimens (273 pus samples + 27 wound swab samples) were collected during the study period, in which 103 samples were collected from male patients and 197 samples were collected from female patients. 
Results: The results showed that 61% were gram-positive and 37% were gram-negative. Samples collected were cultured using standard microbiological techniques and the colonies grown were identified with the help of biochemical tests. The antimicrobial susceptibility testing was performed by the Kirby-Bauer disc diffusion technique. In this study, the frequency of Staphylococcus aureus bacteria was higher. Klebsiella pneumoniae was the predominant Gram-negative bacteria. The penicillin group of antibiotics shows more resistance in most of the organisms. It is essential to establish an accurate schedule for the use of antibiotics and assess the resistance pattern intermittently in each region based on the antibiotic resistance pattern. The detection of infection, control measures and appropriate use of antibiotics must be considered compulsory to decrease wound infection rates and to prevent the further spread of resistance.