Determination of β-carotene in jute leaves by spectrophotometry and thin layer chromatography
1 Microbiology and Biochemistry Department, Technology Wing, Bangladesh Jute Research Institute, Manik Mia Avenue, Dhaka-1207, Bangladesh.
2 Mechanical Processing Division, Technology Wing, Bangladesh Jute Research Institute, Manik Mia Avenue Dhaka-1207, Bangladesh.
3 Jute Seed Production and Research Center, Dinajpur, Bangladesh Jute Research Institute, Bangladesh.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2022, 09(02), 011–020.
Article DOI: 10.30574/wjbphs.2022.9.2.0038
Publication history:
Received on 07 January 2022; revised on 08 February 2022; accepted on 10 February 2022
Abstract:
β-carotene is a carotenoid pigment that converts to vitamin A in human body and plays crucial role in visual functions, reproductive performance and immune system. Jute leaves contain β-carotene but amounts in different varieties of jute leaves are not known. The study was undertaken to estimate the β-carotene content in fresh jute leaves of the cultivated jute species Corchorus olitorius L. and Corchorus capsularis L. by spectrophotometry and thin layer chromatography. β-carotene content varies in leaves of different varieties of two species. Leaves of some C. olitorius variety showed higher amount of β-carotene than the leaves of C. capsularis variety. Some varieties of C. olitorius showed similarity with some varieties of C. capsularis. Variety Robi-1 of C .olitorius showed the highest content of β-carotene 39.93mg/100g. O-72, O-4, O-3820, O-9897 and OM-1 of olitorius variety showed higher β-carotene than all the varieties of C. capsularis. JRO-524 and MG-1 of C. olitorius variety is comparable to CVL-1, D-154, BJC-2197 and deshi pat shak-1 variety of C. capsularis regarding β-carotene content. Lowest β-carotene was estimated 12.55mg/100g in O-795 of olitorius variety and 12.32mg/100g in BJC-83 and 11.34mg/100g in BJC-2142 of capsularis variety. Thin layer chromatography system silica gel on TLC Al foils as stationary phase and hexane as mobile phase performs good separation and identification of β-carotene in jute leaves.
Keywords:
β-carotene; Jute leaves; Corchorus olitorius; Corchorus capsularis; Spectrophotometry; Thin layer chromatography
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