Determination of In-vitro antioxidant activity of Ougeinia oojeinensis leaves

In this investigation, the In-vitro antioxidant Activity of extracts of leaves of Ougeinia oojeinensis was evaluated. The antioxidant activity in vitro was measured by means DPPH, Reducing power and hydroxyl radical scavenging activity. DPPH radical scavenging activity of ethyl acetate extract of Ougeinia oojeinensis exhibited percent inhibition 66.774% and its IC50 value was found to be 43.503μg/ml. DPPH radical scavenging activity of methanol extract of Ougeinia oojeinensis exhibited percent inhibition 76.267% and its IC50 value was found to be 25.722μg/ml. Ascorbic acid was used as a reference compound which exhibited percent inhibition 89.428% and showed IC50 value of 15.615 μg/ml.
Similarly hydroxyl radical scavenging activity of ethyl acetate extract of Ougeinia oojeinensis exhibited percent inhibition 62.183% and its IC50 value was found to be 69.341 μg/ml. Similary, hydroxyl radical scavenging activity of methanol extract of Ougeinia oojeinensis exhibited percent inhibition 75.84% and its IC50 value was found to be 33.360μg/ml. Ascorbic acid was used as a reference compound which exhibited percent inhibition 87.749% and showed IC50 value of 9.904 μg/ml. The methanolic extract showed strong reducing capacity as compared to ethyl acetate extract of Ougeinia oojeinensis.