Effects of plant growth regulators on virus-free plantlet regeneration from Abroma augustum (L.) seeds

Md. Enamul Haque 1, Homayra Binta Sujaye 2, Biswanath Sikdar 2. Uzzal Kumar Acharjee 2, Md. Khalekuzzaman 2 and Md. Faruk Hasan 3, *

1 Department of Biotechnology and Genetic Engineering, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, P.O. Box 8100, Dhaka, Bangladesh.
2 Department of Genetic Engineering and Biotechnology, University of Rajshahi. P.O. Box 6205, Rajshahi, Bangladesh.
3 Department of Microbiology, University of Rajshahi, P.O. Box 6205, Rajshahi, Bangladesh.
 
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 19(03), 176–183.
Article DOI: 10.30574/wjbphs.2024.19.3.0617
Publication history: 
Received on 30 July 2024; revised on 08 September 2024; accepted on 11 September 2024
 
Abstract: 
Abroma augusta is a popular folk therapeutic plant in Bangladesh, rightly called Devil’s cotton in English. The present study was performed to evaluate the in vitro multiple shoot and root generation of Abroma augustum plants. In vitro seed germination, shoot proliferation and root induction were performed following previous methods. The highest percentage of seed germination was 90±0.0% after seven days of inoculation, after which the plants were sterilized with 0.1% NaOCl for 2 min. The longest shoot length was 7.6±0.6 cm at the MS+2+0.3 mg/L BAP+NAA concentration. The greatest number of roots per shoot was 12.0±1.0 cm, and the greatest length of roots was 6.8±1.6 cm in MS media supplemented with 1.0 mg/L IBA. Both the shoots and roots were vigorous and sufficient in length and number. The present study established an efficient in vitro micropopagation process for Abroma augustum plantlet production through seeds. The findings of the present research will be helpful for rapidly regenerating disease-free plantlets.
 
Keywords: 
Abroma augustum; Seeds; In vitro regeneration; Shoot proliferation; Root proliferation
 
Full text article in PDF: 
 
Copyright information: 
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0