Hepatotoxicity evaluation of an aqueous extract of Clinopodium vimineum (L.) Kuntze in female Wistar rats

Katherine Morales-Torres 1, Juan José Mora-Román 2, 3, José Manuel Fallas-Ramírez 2, 3, Jesús Alexander Rodríguez-Arrieta 4, 5 and Josué Orozco-Aguilar 3, 5, *

1 Faculty of Pharmacy, Universidad de Costa Rica, San José, Costa Rica.
2 Industrial Pharmacy Department, Faculty of Pharmacy, Universidad de Costa Rica, San José, Costa Rica.
3 Instituto de Investigaciones Farmacéuticas (INIFAR), Faculty of Pharmacy, Universidad de Costa Rica, San José, Costa Rica.
4 Research Center in Microscopic Structure (CIEMic), Universidad de Costa Rica, San José, Costa Rica.
5 Pharmacology, Toxicology, and Pharmacodependence Department, Faculty of Pharmacy, Universidad de Costa Rica, San José, Costa Rica.
 
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 13(03), 197–201.
Article DOI: 10.30574/wjbphs.2023.13.3.0124
Publication history: 
Received on 30 January 2023; revised on 10 March 2023; accepted on 13 March 2023
 
Abstract: 
Clinopodium vimineum has been used to relieve gastrointestinal symptoms due to its carminative and antispasmodic properties. Besides, it has been associated with antimicrobial, sedative, analgesic, anti-inflammatory, and healing effects. Nevertheless, its essential oil has hepatotoxic compounds such as pulegone, so the plant's possible hepatotoxic effect on aqueous extract was evaluated with a female Wistar rats model. An aqueous extract of the vegetal material was prepared, and an assay was carried out with three experimental groups of five subjects each. The first consisted of a control group that did not receive the extract; the second was treated for four days with the extract, receiving a loading dose on the first day. The third was similar to the previous one, except for not receiving the indicated loading dose. Each animal was evaluated for some hepatic function parameters (alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase). Likewise, histopathological analysis of the liver was performed. With the information obtained, it was impossible to establish that the aqueous extract of C. vimineum has a hepatotoxic effect in female Wistar rats.
 
Keywords: 
Clinopodium vimineum (L.) Kuntze; Aqueous extract; Wistar rats; Hepatic function parameters; Histopathological analysis.
 
Full text article in PDF: 
 
Copyright information: 
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0