HPLC method development and validation for the estimation of methyl prednisolone in marketed formulation
1 Technocrats Institute of Technology Pharmacy, BHEL Bhopal Madhya Pradesh, India.
2 TIT College of Pharmacy, BHEL Bhopal Madhya Pradesh, India.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 20(01), 276–288.
Article DOI: 10.30574/wjbphs.2024.20.1.0410
Publication history:
Received on 01 June 2024; revised on 12 July 2024; accepted on 15 July 2024
Abstract:
In the present research work, a successful attempt was made for “Method, development and validation for the estimation of drug in marketed formulation” which was developed by experimentation based on thorough literature survey and ascertained by statistical parameters of sampling. The simplicity, rapidity, accurate and reproducibility of the proposed methods completely fulfill the objective of the research work of estimation of the drug in marketed formulation. HPLC method was found to be linearin the range of1- 5 μg/ml methyl prednisolone with the correlation coefficient near to one (0.999) respectively. The validation and the reliability of proposed method were as sessed by recovery study. The recovery of added standards (80%, 100% 120%) was ranging near to one for atorvastatin and clopidogrel respectively. Liquid chromatographic system from waters comprising of manual injector, Waters 515 binary pump for constant flow and constant pressure delivery and U.V. detector connected to data ace software controlling the instrumentation as well as processing the data generated were used. The is ocratic mobile phase consisted of Methanol: Acetonitrile in the ratio of 50:50v/vat a flow rate of 1.0ml min-1. A thermo C-18 column (4.6 x 250mm, 5μ particle size) was used as the stationary phase, 254.0 nm was selected as the detection wave length for UV-vis. Detector.
Keywords:
HPLC; Methyl prednisolone; Acetonitrile; Atorvastatin; Reproducibility
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