Interaction study of 5- Fluorouracil with Bovine serum albumin by UV-Visible spectrophotometry
Department of pharmaceutical analysis, ELIMS College of pharmacy, Thrissur, India.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 15(02), 143–150.
Article DOI: 10.30574/wjbphs.2023.15.2.0343
Publication history:
Received on 01 July 2023; revised on 13 August 2023; accepted on 16 August 2023
Abstract:
Introduction: 5-Fluorouracil (5-FU) is primary chemotherapeutic drug used for most colorectal, pancreatic and aggressive forms of breast cancers. Serum albumin is major carrier protein and its binding with drugs is important to examine the change in pharmacokinetic properties which directly influence the bio availability, sustained drug release and toxicity of drugs.
Aim and objectives: The aim of the project is to perform a study on interaction of 5 - fluorouracil with bovine serum albumin employing the UV-Visible spectrophotometric method.
Methods: Interaction of 5-FU with BSA has been studied systematically using UV spectroscopy by fixing the carbonate buffer of pH 7.4 as solvent and at a suitable wavelength 230 nm and 275 nm. To gain an insight into the interaction of 5-FU with BSA, the spectra of the 5-FU and BSA were examined in the absence and presence of various concentrations of the 5-FU and BSA respectively at zero minutes and repeated in 30 minutes, 60 minutes and 90 minutes time interval. The changes in peak intensity, absorbance and shift in peaks were observed. The detectable differences were recorded. This method can be used successfully for the interaction study of 5-FU with BSA.
Results: Interaction study of 5-Fluorouracil with bovine serum albumin revealed that appreciable changes in absorbance, peak area and shifts in peak were observed which may be due to binding of drug with protein BSA.
Keywords:
5-Fluorouracil; Bovine serum albumin; Chemotherapeutic; UV visible spectroscopy; Interaction study
Full text article in PDF:
Copyright information:
Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0