To investigate the anti-bacterial activity of crude extracts of Mintha pipertia and Allium sativum against throat infection causing bacteria streptococci collected from various diagnostic laboratories and hospitals of Mingora swat
1 Institute of Microbiology, University of Sindh, Jamshoro, Pakistan.
2 Pharmaceutical Research Centre, PCSIR Laboratories Complex, Karachi, Pakistan.
3 Food and Marine Resource Research Centre, PCSIR Laboratories Complex, Karachi, Pakistan
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 15(01), 066–074.
Article DOI: 10.30574/wjbphs.2023.15.1.0289
Publication history:
Received on 20 May 2023; revised on 09 July 2023; accepted on 12 July 2023
Abstract:
Streptococcus (S) pyogenes one of the major Gram-positive pathogens responsible for upper respiratory tract and nosocomial infections. This is a Gram Positive, aerobic, no motile, and non-spore-forming ubiquitous bacteria. The present study was conducted to evaluate the in vitro antibacterial activity of ethanol and aqueous extract of peppermint and garlic against 29 isolates of S. pyogenes recovered from the throat sample of patientsin Swat KPK-Pakistan. These isolates were purified to identify the target bacterial isolates by gram staining and biochemical tests. The Agar dilution and well method was applied for the antibacterial activity of garlic and peppermint extracts. All of the subject isolates were exposed to different concentrations of peppermint. MIC of aqua extract of peppermint was 250µL/mL and ethanol extract was 100µL/mL. The zone size was 29mm and 36mm respectively. So the ethanol extract of peppermint was found to be more effective as compared to the aqueous extract. On the other hand, Garlic has shown equal effectiveness of water and ethanol extraction with MIC value of all is 50µL/mL and zone of inhibition was 38mm. Ciprofloxacin antibiotic disk was used as reference standard.
Keywords:
Streptococcus pyogenes; Mentha piperita L; Allium sativum; Antibacterial activity
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