Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients Molecular detection of carbanemase in gram negative bacteria Isolated from intensive care unit patients in wasit province, Iraqin wasit province, Iraq
1 Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq.
2 Department of Pediatric, Faculty of Medicine, University of Wasit, Iraq.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2022, 12(02), 130-139.
Article DOI: 10.30574/wjbphs.2022.12.2.0177
Publication history:
Received on 28 September 2022; revised on 12 Novemebr 2022; accepted on 15 November 2022
Abstract:
ICUs are crucial medical facilities for treating life-threatening diseases, and bacterial contamination of ICU equipment and devices is a significant cause of nosocomial infections. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. 30(36.5%) were female and 52( 63.5%) were male patients admitted to the intensive care unit distributed 69 (84.1%) gram-negative bacteria .while gram positive 13(15.8%), as well as 20 pure isolates of some places, materials and equipment distributed 12 (75%) Gram negative bacteria .While 8 (25%) it is gram positive bacteria. Results of antibiotic susceptibility test only Trimethoprime was effective choice against Acinetobacter baumannii. Phenotypically survey of carbapenem klebsiella pneumoniae were 9(47%), pseudomonas aeruginosa was 7(35% of MBL and Carbapenemase and the result of MBL and Carbapenemase for E. Coli 7(43%) similar results (40%). Whereas, Genotypically the most prevalent gene in E.Coli 1 (10%), the result was for Burkholderia cepacia blaVIM 1(100%), its prevalence was 7 (53%) among bacteria Klebsiella pneumonia for NDM, the result of bacteria Pseudomonas aeruginosa for this gene was 4(33.3%) and Escherichia Coli was 1(10%), its prevalence was (100%) among klebsiella oxytoca for KPC.
Keywords:
Intensive care unit; Bacteria characterization; Antibiotic susceptibility Phenotypes and genotype of MBL; Carbapenemase gram negative bacteria
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