Molecular detection of diarrheagenic Escherichia coli pathotypes isolated from drinking water from rural communities in Wamba, Nasarawa State, Nigeria
Department of Microbiology, Faculty of Natural and Applied Science, Nasarawa state university Keffi, Nasarawa state, Nigeria.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 20(02), 001–006.
Article DOI: 10.30574/wjbphs.2024.20.2.0848
Publication history:
Received on 19 September 2024; revised on 28 October 2024; accepted on 31 October 2024
Abstract:
Contamination of drinking water with pathogenic Escherichia coli (E.coli) poses significant health risks, particularly in rural communities. This study was carried out to detect diarrheagenic E.coli pathotypes isolated from drinking water in three selected rural communities in Wamba, Nasarawa state, Nigeria. Fifteen drinking water samples comprising of stream (5), well (5) and borehole (5) were collected. E.coli was isolated by streaking on Eosin Methylene Blue agar. Typical green colonies with metallic sheen were Gram stained and tested biochemically. The isolates were analyzed molecularly using multiplex Polymerase chain reaction (PCR) technique with specific primers and were found to be carrying Vt2, eaeA and estA genes associated with EHEC, EPEC and ETEC pathotypes respectively. The agarose gel electrophoretic assay was used for the detection of amplified genes for different diarrheagenic E.coli pathotypes. The isolation of this organism possessing virulence genes from drinking water is of public health significance and therefore more attention needs to be paid to the drinking water of Wamba, Nasarawa state.
Keywords:
Escherichia coli; Virulence genes; Drinking water; Polymerase chain reaction; Pathotypes.
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