Molecular detection of extended spectrum β-lactamase in gram negative bacteria isolated from intensive care unit patients in Wasit province, Iraq
1 Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq.
2 Department of Pediatric, Faculty of Medicine, University of Wasit, Iraq.
Review
World Journal of Biology Pharmacy and Health Sciences, 2022, 12(02), 094-104.
Article DOI: 10.30574/wjbphs.2022.12.2.0178
Publication history:
Received on 23 September 2022; revised on 12 November 2022; accepted on 15 November 2022
Abstract:
Intensive care units (ICU) are epicenters for the emergence of antibiotic-resistant Gram negative bacteria and multi-resistant Gram positive infections, largely due to the inappropriate use of antimicrobials. In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture. The findings of the disc diffusion method used to test the isolates for antibiotic susceptibility showed that Amikacin was the most effective antibiotic against klebsiella pneumonia and pseudomonas aeruginosa. Phenotypically survey of ESBLs for all isolates were ESBL producers, of these, 13(68.4%) isolates were determined as ESBLs-producers following confirmatory testing to be ESBLs producers. Whereas, genotypically no isolate had ESBLs producers CTX-M-type ESBL. The prevalence of TEM, SHV, and OXA-1 were as follow: blaSHV 3(23 %). While blaTEM and blaOXA-1genes were absent among all isolates. Phenotypically survey of ESBLs, for (20) isolates pseudomonas aeruginosa 20(100%) isolates were ESBLs producers of these, 12(60%) isolates were determined as ESBLs-producers following confirmatory testing to be ESBLs producers. While genotypically isolates were ESBL producers 1(8.3%) of the isolates were ESBLs producers CTX-M-type ESBL was the most prevalent CTX-M2 was 1(8.3%) , CTX-M1 and CTXM9 were absent among all isolates. The prevalence of TEM, SHV, and OXA-type1 were as follow: blaSHV 4(33%). While blaTEM and blaOXA-1 genes were absent among all isolates. Phenotypically survey of ESBLs, for (17) isolates E. coli 17(100%) isolates were ESBLs producers of these, 10(58.8%) isolates were determined as ESBLs-producers following confirmatory testing to be ESBLs producers. Whereas, Genotypically no isolate ESBLs producers CTX-M-type ESBL, prevalence of TEM, SHV, and OXA-1 were as follow: blaSHV 1(10%),blaOXA-1 1(10%) . While blaTEM gene was absent among all isolates.
Keywords:
Antibiotic susceptibility; ESBLs; Gram negative bacteria; Intensive care unit
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