Peripheral blood transcriptome analysis in sepsis-associated acute kidney injury
1 Cangnan Hospital of Traditional Chinese Medicine, Wenzhou 325800, Zhejiang, China.
2 The Affiliated Cangnan Hospital of Wenzhou Medical University, Wenzhou 325800, Zhejiang, China.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 19(03), 438–444.
Article DOI: 10.30574/wjbphs.2024.19.3.0663
Publication history:
Received on 05 August 2024; revised on 18 September 2024; accepted on 20 September 2024
Abstract:
Sepsis-associated acute kidney injury (S-AKI) presents significant clinical challenges, necessitating deeper insights into its molecular mechanisms. We conducted a comprehensive analysis—including Principal Component Analysis (PCA), differential gene expression, KEGG pathway enrichment, and Gene Set Enrichment Analysis (GSEA)—to elucidate the underlying pathways involved in S-AKI. PCA revealed distinct clustering between S-AKI patients and controls, with differential gene expression identifying 1,759 significant genes (912 upregulated, 847 downregulated). Notable upregulated genes included CYP19A1 and LY6G6F, while downregulated genes encompassed LRP1B and SIGLEC8. KEGG analysis highlighted pathways related to adaptive immune responses and infection, such as “Th1 and Th2 cell differentiation" and "Primary immunodeficiency.” GSEA pinpointed "Neutrophil extracellular trap formation" as the most significantly enriched pathway, underscoring neutrophil-mediated responses in S-AKI. Our findings suggest potential molecular targets for diagnosis and therapeutic intervention, providing a foundation for future research and improved clinical outcomes.
Keywords:
Sepsis; Acute Kidney Injury; Transcriptome; Inflammation
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Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0