Phylogenetic tree analysis based on the 16S sequence alignment for Proteus mirabilis Isolated from UTI patients in Wasit province, Iraq
1 Department of Medical Microbiology, Faculty of Medicine, University of Wasit, Iraq.
2 Department of Medicine, Faculty of Medicine, University of Wasit, Iraq.
3 Department of Biology, Faculty of Education for pure science, University of Kerbala, Iraq.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2022, 12(01), 125–132.
Article DOI: 10.30574/wjbphs.2022.12.1.0154
Publication history:
Received on 07 September 2022; revised on 14 October 2022; accepted on 17 October 2022
Abstract:
One of the most common bacterial illnesses worldwide is urinary tract infections (UTIs). Out of 228 urine specimens collected from a patient with suspected UTIs, 40 (17.5%) Proteus mirabilis isolates were identified, the isolates were first identified as related to culture, microscopic examination, and biochemical tests. DNA was extracted from these 40 isolates, the sample's nucleic acid purity ranged from (1.8-2) while its concentration was between (50 - 360 mg/ul).
Isolates were detected as Proteus mirabilis by molecular methods of 16S rRNA gene for confirmatory diagnosis and phylogenetic tree. In the current study the phylogenetic tree analysis showed the significant selection potential of 16S rRNA to identify isolated Proteus mirabilis and the close with Iraqi strain.
Keywords:
Proteus mirabilis; Urinary tract infections; Phylogenetic tree; Vitek 2; PCR
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