Production of Bioplastic (Polyhydroxybutyrate) by Micrococcus luteus isolated from water sample
Department of Microbiology, Maharani Cluster University, Bangalore -560001, India.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 20(02), 960–973.
Article DOI: 10.30574/wjbphs.2024.20.2.0932
Publication history:
Received on 15 October 2024; revised on 27 November 2024; accepted on 30 November 2024
Abstract:
Polyhydroxybutyrate (PHB) is a biodegradable polymer produced by various microorganisms as a storage material, offering a sustainable alternative to traditional plastics. Biodegradability of Bioplastics has been widely publicized in society and the demand for packaging is rapidly increasing among retailers and the food industry at large scale. The plastic which is available in market is very dangerous as it is nonbiodegradable. Therefore, it is the demand of the day that biodegradable plastics should be produced and used. This study investigates the production and optimization of PHB using Micrococcus luteus, a gram-positive bacterium known for its ability to synthesize PHB under suitable conditions. The research focuses on optimizing culture conditions, including the selection of carbon and nitrogen sources, pH, and temperature, to maximize PHB yield. Micrococcus luteus was cultured in a basal medium with different carbon sources (glucose, fructose, sucrose, maltose) and nitrogen sources (ammonium sulphate, peptone, yeast extract, urea). The optimal conditions were determined through systematic variation of pH (5 to 9) and temperature (20°C, 30°C, 40°C and 50ᵒC). This study will also spotlight Micrococcus luteus as a potential source for large scale production of PHB with reducing environmental pollutions. Thus, the bacterial mediated PHB synthesis can be used as a better alternative to deal with the currently available practices of plastic use.
Keywords:
Polyhydroxybutyrate; Micrococcus luteus; Sudan black B stain; Sodium hypochlorite method; Optimization; Growth curve.
Full text article in PDF:
Copyright information:
Copyright © 2024 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0