A rapid HPTLC method to estimate ximenynic acid in semisolid dosage formulation
1 Research Scholar, Department of pharmaceutical sciences, JJT University, Jhunjhunu- 333001, Rajasthan, India.
2 Vice president, Clinical Research, Mprex Healthcare, Pune, Maharashtra, India.
3 Research Guide, Department of pharmaceutical sciences, JJT University, Jhunjhunu- 333001, Rajasthan, India.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 13(02), 080-088.
Article DOI: 10.30574/wjbphs.2023.13.2.0085
Publication history:
Received on 16 November 2022; revised on 05 February 2023; accepted on 07 February 2023
Abstract:
A novel HPTLC analytical technique and validation study were developed to determine the concentration of ximenynic acid (XMA) in semisolid dosage formulations (SDF) such as cream, gel, lotion, etc. The procedure makes use of reverse-phase high-performance liquid chromatography (HPLC). This study presents the first report of sensitive, selective, precise and robust HPTLC method, which has been developed and validated for quantification of the XMA from pharmaceutical formulation. The chromatographic development was carried out on HPTLC plates precoated with silica gel 60 F254 using a mixture of Toluene: Ethyl Acetate: Methanol: Formic acid (5:4:0.5:0.5 v/v/v/v) as mobile phase. Detection was carried out densitometrically at 254 nm. The Rf value of XMA were found at Rf about 0.3 ± 0.01. The method was validated as per ICH guideline with respect to linearity, accuracy, precision, robustness etc. The method is new, simple and economic for routine estimation of XMA in bulk, preformulation studies and pharmaceutical formulation to help the industries as well as researchers for their sensitive determination of XMA rapidly at low cost in routine analysis. Because XMA has the potential to be a game-changing method of treating ageing, its advancement may benefit the pharmaceutical and cosmetics sectors.
Ximenynic acid; Skin Care Medicinal Formulation; HPTLC; Validation
Keywords:
Ximenynic acid; Skin Care Medicinal Formulation; HPTLC; Validation
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