Department of Pharmaceutical Analysis, Malla Reddy Institute of Pharmaceutical Sciences, Maisammaguda, Kompally. India.
World Journal of Biology Pharmacy and Health Sciences, 2025, 21(01), 282-289
Article DOI: 10.30574/wjbphs.2025.21.1.0018
Received on 30 November 2024; revised on 08 January 2025; accepted on 10 January 2025
Methylcobalamin, a vital vitamin B12 analog, is susceptible to degradation under thermal stress, leading to changes in PH. Trigeminal neuralgia, diabetic neuropathy, facial paralysis and megaloblastic anemia can be treated by the use of Methylcobalamin injection. A new simple, precise, economically viable and efficient High-Performance Liquid Chromatographic (HPLC) method for the estimation of Methylcobalamin (MeB12) in bulk drugs and pharmaceutical dosage formulations was developed and validated. The method was validated for linearity, accuracy, precision, LOD, LOQ and robustness as per ICH guidelines. The method was tested with commercially available MeB12 drugs and the concentration methylcobalamine was found to be accurate.
Methyl cobalamin; HPLC; Method validation; Vitamin
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Krishnaphanisri Ponnekanti, Addanki Anusha, Geetha Jummidi, Sai Halavathu and Shivareddy Karnati. A review: On HPLC method for estimation of methylcobalamin. World Journal of Biology Pharmacy and Health Sciences, 2025, 21(01), 282-289. Article DOI: https://doi.org/10.30574/wjbphs.2025.21.1.0018.