Review on method development and validation of pharmaceutical dosage form by using UV visible spectroscopy and HPLC

UV visible Spectroscopy is a form of absorption spectroscopy; Spectroscopy is often used in physical and analytical chemistry for the identification of substance through the spectrum emitted from or absorbed by them. Spectroscopy is also heavily used in astronomy and remote sensing. Most large telescopes have spectrometer, which are used either to measure the chemical composition and physical properties of astronomical objects of to measure the velocities from the Doppler shift of their spectral lines.
High performance liquid chromatography (HPLC) is an important qualitative and quantitative technique, generally used for the estimation of pharmaceutical and biological sample. It is the safest versatile, dependable and fastest chromatographic technique for the quality control of the drug component.