Screening and molecular identification of l-asparaginase producing bacteria with ansB gene from sewage water
1 Department of Biotechnology Barkatullah University Bhopal, India.
2 Department of Botany and Biotechnology, GOVT MGM College Itarsi, India.
3 Department of Biotechnology Barkatullah University Bhopal, India.
4 Department of Biotechnology St. Aloysius College Jabalpur, India.
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2024, 18(02), 058–064.
Article DOI: 10.30574/wjbphs.2024.18.2.0234
Publication history:
Received on 17March 2024; revised on 28 April 2024; accepted on 01 May 2024
Abstract:
Enzymes are vital biocatalysts facilitating biochemical reactions in organisms, playing pivotal roles in both biological systems and numerous industrial applications. This study focuses on the isolation and screening of asparaginase-producing bacteria from sewage water samples in Bhopal City. A modified M9 media, supplemented with asparagine and phenol red, was used to isolate asparaginase-producing bacteria. Out of the ten samples, seven exhibited asparaginase activity. Two bacterial strains, identified as Bacillus tropicus (S-1) and Priestiaveravalensis (S-2), were found to harbor the ansB gene. Sequence analysis revealed a 96.52% and 95.09% resemblance to Bacillus tropicus MCCC 1A01406 and Priestiaveravalensis strain SGD-V-76, respectively. These findings highlight the potential of these bacterial strains in producing L-asparaginase, emphasizing their significance in pharmaceutical applications, particularly in cancer treatment.
Keywords:
Anticancer enzymes; L-Asparaginase; Sewage water; Microbial metabolites
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