1 The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, UAE.
2 International Center for Forensic Sciences, Dubai Police General Head Quarters, Dubai, UAE.
3 School of Law and Policing, University of Central Lancashire, Preston, UK.
4 Department of Medical, Surgical and Advanced Technologies “G.F. Ingrassia”, University of Catania, Italy
World Journal of Biology Pharmacy and Health Sciences, 2025, 21(03), 234-244
Article DOI: 10.30574/wjbphs.2025.21.3.0270
Received on 29 January 2025; revised on 07 March 2025; accepted on 10 March 2025
This study investigates the persistence of touch DNA on human skin to enhance forensic methodologies in violent crime investigations. Touch DNA, a critical piece of forensic evidence, can link suspects to crimes through genetic material recovered from skin surfaces. However, its recovery presents challenges, particularly regarding the temporal window for effective collection and the impact of environmental factors on DNA preservation. Through a controlled experimental setup, this research examined how the quantity and detectability of touch DNA change over time, with a focus on the effects of post-deposition activities such as washing. The findings reveal that while DNA quantity increases over time, recovering alleles from mixed DNA profiles—comprising both victim and perpetrator DNA—becomes increasingly difficult. Notably, alleles from the perpetrator become undetectable after 72 hours (p < 0.05), and environmental interactions, such as washing or sweating, further complicate recovery. This study underscores the importance of timely evidence collection and proposes considerations for forensic protocols to optimize touch DNA recovery and analysis. It also highlights the need for further investigation into how skin type and individual DNA shedding rates affect touch DNA persistence. Ultimately, this research contributes valuable insights that can improve the resolution of violent crime investigations through more effective DNA recovery strategies.
Forensic science; Trace DNA; Touch DNA; DNA recovery; Cotton swab; QIAamp DNA investigator kit; Quantifiler™ Human DNA Quantification Kit; GlobalFiler™ PCR Amplification Kit
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Salem K. Alketbi and Luigino Carta. Uncovering the Persistence of Touch DNA on human skin and its implications for violent crime investigations. World Journal of Biology Pharmacy and Health Sciences, 2025, 21(03), 234-244. Article DOI: https://doi.org/10.30574/wjbphs.2025.21.3.0270.