Verification of the Lactate Dehydrogenase dosage method using Abbott Architect ci8200 experience of the biochemistry laboratory, CHU Mohammed VI Oujda, Morocco

Introduction: This work describes the verification procedure to evaluate the analytical performance of lactate dehydrogenase (LDH) assay, a key enzyme in glycolysis. It catalyzes the reversible conversion of pyruvate to lactate, with simultaneous production of NADH from NAD⁺. This evaluation forms part of a comprehensive process to validate the methods used in the central laboratory of the Mohammed VI University Hospital in Oujda, with the objective of assembling an accreditation file that meets the NF ISO 15189 standard requirements.

Objectives: The aim of this study was to validate the analytical method for lactate dehydrogenase determination by evaluating key criteria such as repeatability and reproducibility. This validation process sought to ensure that the method complies with the required standards and delivers reliable and accurate results for clinical diagnostics.

Results and Discussion: The verification of the LDH assay criteria demonstrated satisfactory repeatability across all three levels, with CV1=1.34%, CV2=0.71% and CV3=0.60% respectively. Similarly, intra-laboratory reproducibility was deemed satisfactory for all levels, with CV1 = 3.06%, CV2 = 2.50%, and CV3 = 1.77% for the respective levels.

The verification of an analytical method is a critical step in ensuring that the obtained results closely align with the reference values of the sample. A comparison of our findings with the CV standards set by the SFBC (a quality control system) and RICOS (an international quality control network) indicates that our results comply with, and remain below, the acceptable limits.

Conclusion: By implementing rigorous analytical performance verification, laboratories guarantee reliable clinical outcomes. This study enhances the existing knowledge base regarding the accuracy and reliability of serum LDH measurements.