Development and validation of spectroscopic simultaneous equation method for simultaneous estimation of Azelnidipine and Valsartan in synthetic mixture

Harshad Kumar K Patel 1, *, Vineet C Jain 2, Zarana Dedania 1, Naimuddin Riyazuddin Saiyed 1 and Rajendra C Patel 1

1 Department of Pharmaceutical Science, Bhagwan Mahavir University, BMEF Campus, Nr. Aakash E-Space, Bharthana, Vesu, Surat-395017, India.
2 Bhagwan Mahavir University, BMEF Campus, Nr. Aakash E-Space, Bharthana, Vesu, Surat-395017, India.
 
Research Article
World Journal of Biology Pharmacy and Health Sciences, 2023, 13(02), 277–287.
Article DOI: 10.30574/wjbphs.2023.13.2.0042
Publication history: 
Received on 06 December 2022; revised on 26 January 2023; accepted on 28 January 2023
 
Abstract: 
A simple, specific, accurate and precise spectrophotometric method was developed and validated for simultaneous estimation of Azelnidipine and Valsartan in Synthetic Mixture. The wavelength of estimation for Azelnidipine was 240.00 nm and for Valsartan was 250.00 nm. Beer’s law is obeyed in the concentration range of 2-10μg/ml and 16-80μg/ml and correlation coefficient of 0.9999 and 0.9993 for Azelnidipine and Valsartan respectively. The % recovery for Azelnidipine and Valsartan were found to be 101.2 % - 101.9 % and 101.8 - 101.9 % respectively. Intraday precision of Azelnidipine and Valsartan were found to be 0.06 – 0.24 and 0.08 – 0.27 % RSD and Interday precision were found to be 0.14 – 0.33 and 0.11 – 0.37 % RSD respectively. The proposed method was also evaluated by the Assay of Synthetic mixture containing Azelnidipine and Valsartan. The % Assay was found to be 100.50 ± 0.002 for Azelnidipine and 100.87 ± 0.001 for Valsartan. Validation of proposed methods was carried out according to ICH Q2R1Guidelines. The proposed methods were found accurate and reproducible for routine analysis of both the drugs in synthetic mixture.
 
Keywords: 
Azelnidipine; Valsartan; Simultaneous equation method; UV Spectroscopy
 
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Copyright © 2023 Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution Liscense 4.0