Simultaneous determination and validation of Aceclofenac and cyclobenzaprine hydrochloride by area under curve and q-absorbance ratio methods in bulk and pharmaceutical formulations

A simple, accurate, rapid and UV spectrophotometric methods has been developed for the simultaneous estimation of Aceclofenac (ACF) and Cyclobenzaprine hydrochloride (CBP) in bulk and combined tablet dosage form. The methods employed were(A) Area under curve and (B) Q-absorbance ratio method. Method- A involves measurement of peak area in the wavelength range 271-281 nm and 220-230 nm for ACF and CBP respectively. In Method B two wavelengths 276 nm, λ max of Aceclofenac and 257 nm, the isobestic point were selected. In both the methods linearity was found in the concentration range of 6-30 µg/ml for Aceclofenac and 0.45-2.25 µg/ml Cyclobenzaprine hydrochloride. Both the methods were found to be rapid, specific, precise and accurate. Hence these methods can be applied for routine analysis of Aceclofenac and Cyclobenzaprine Hydrochloride in combined dosage form without any interference by the excipients. The above methods are validated according to ICH guidelines.