Formulation and evaluation of topical anti-microbial herbal cream

Background: The aim of this study was to determine the antimicrobial effect of neem oil with liquid paraffin and coconut oil and their combination on the bacteria E. coli, S. auerus and Candida albicans, which causes skin diseases ,increasingly resistant to many commonly used antibiotics as a result of colonization of the skin.
Methods: Nine different emulsions were prepared and evaluation were carried out including Homogeneity, FTIR,Phytochemical tests, Drug content and In-vitro drug release and Anti-Microbial studies were performed.
Results: The results revealed that the Phyto chemical Screening of Neem oil found to be presence of Alkaloids, glycosides, Saponins, flavonoids pH of the cream was found to be in the range of 5.8-6.7 which is good for the skin pH. All the formulation were near to the skin pH. The viscosity of the cream was found 28008-28932 cps.
Conclusion: Phyto chemical Screening of Neem extract were done and found to be presence of Alkaloids, glycosides, Saponins, flavonoids etc were present. IR studies revealed that there was no drug Excipients interaction. During our physicochemical evaluation studies all the formulation were found to have good ,pH and Viscosity,diffusion .In-vitro drug release studies showed that, the formulation F9 showed optimum drug release 97.17±2.000 in 3 hours. The tested organisms, particularly gram positive, gram-negative organisms and Fungi Candida albicans had shown high resistance towards different Antibiotics, Thus neem oil is effective against drug resistant organisms.