Impact of oxidative stress induced by chlorpyrifos in male rats: The ameliorating effect of rutin and vitamin E

The present study was designed to assess some toxicological effects in male rats following exposure to technical and formulated chlorpyrifos (CPF T. and CPF F.; at a dose of 10 mg a.i. kg-1 b.wt.) and to evaluate the possible ameliorative effect of Rutin (RT, 50 mg/kg) or Vitamin E (Vit.E, 100 mg/kg) administration. Nine groups of rats were designated for: control; [Vit.E]; [RT]; [CPF.T]; [CPF T.+Vit.E]; [CPF T.+RT]; [CPF F.]; [CPF F.+Vit.E] and [CPF.F+RT] treatments. Administration of CPF T. and CPF F. caused a significant increase in (LPO) level and decrease in the activities of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD), glutathione-s-transferase (GST), Glutathione peroxidase (GPx), and glutathione reductase (GR). Also, it caused a significant increase in serum aminotransferases, alanine transaminase (ALT) and aspartate transaminase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine and uric acid levels, whereas albumin, total protein and cholinesterase (ChE) were decreased. In contrast, the present results demonstrated that co-administration of Vit.E or Rutin minimized oxidative damage included by CPF  as the levels of LPO, CAT, SOD, GST, GPx, GR, AST, ALT, ALP, LDH, creatinine, uric acid, ChE, albumin and total protein were maintained to levels not significantly different from that of the normal control. These results suggest the protective role of Vit.E and Rutin in alleviating the oxidative stress and the toxicity induced by CPF in male adult rats.