Simultaneous estimation of naltrexone and bupropion in pharmaceutical dosage form by using UV spectroscopy

A sensitive and validated method has been developed for simultaneous estimation of Naltrexone and Bupropion in pharmaceutical dosage form by using UV Spectroscopy, without prior separation, by four different techniques (Simultaneous Equation, Absorbance Ratio method, Dual Wavelength Method and Derivative Spectroscopic Method).  The work was carried out on Shimadzu electron UV1800 double beam UV-Visible spectrophotometer. The absorption spectra of reference and test solutions were carried out in 1 cm matched quartz cell over the range of 200 - 400 nm. The linearity ranges for Naltrexone and Bupropion were 2-10 μg/ml. The results of the analysis have been validated statistically and by recovery studies. The proposed procedures are rapid, simple, require no preliminary separation steps and can be used for routine analysis of both drugs in quality control laboratories.