Detection of Myogenic Factor 5 (MYF5/TaqI) gene monomorphism in Bali Cattle (Bos javanicus)

Bali cattle (Bos javanicus) are the indigenous cattle of Indonesia that kept for meat production purpose. This study was aimed to observe the genetic diversity in the Myogenic Factor 5 (MYF5) gene with PCR-RFLP analysis. Thirty-one (31) heads of Bali cows from Klungkung Regency, Bali Province were used for the investigation. Notably, PCR-RFLP analysis was performed to detect the mutation site g.1948A>G in the intron 2 of bovine MYF5/TaqI gene. Nonetheless, no polymorphism in the MYF5/TaqI gene of Bali cattle. Hence, all animals under study were identified as GG genotype. Despite this, two mutation sites of g.1707T>C and g.1795C>T in the MYF5 gene were occurred in Bali cattle and not observed in other bovidae species. Furthermore, the intron 2 sequence of MYF5 gene in Bali cattle is closed to Bos frontalis and Bos grunniens. In conclusion, the MYF5/TaqI gene in Bali cattle was monomorphic. In the future, in-dept study to investigate the other regions of MYF5 gene is important to obtain the genetic markers of growth traits in Bali cattle in the future.